Human Anti-FZD5 Recombinant Antibody (PABJ-0145)
CAT#: PABJ-0145
This product is a Human antibody that recognizes Human FZD5. This antibody bound to FZD1, -2, -4, -5, -7, and -8 on the cell surface, but did not bind FZD3, -6, -9, or -10. It showed cross-reactivity to mouse FZD4 and FZD5.
Gene Expression
Tested Data
Normal Tissue
Figure 1 IHC staining of human caudate
Immunohistochemical staining of human caudate shows moderate positivity in neurons.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52361/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52361/127650_B_7_5.jpg
Normal Tissue
Figure 3 Pancreas
Exocrine glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52361/127650_A_2_3.jpg
Normal Tissue
Figure 4 Testis
Elongated or late spermatids Staining: Low Intensity: Moderate Quantity: <25% Pachytene spermatocytes Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52361/127650_A_6_6.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (BEWO, CACO-2, Hep G2, NTERA-2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000163251-FZD5
❮
❯
SDS-PAGE
Figure 1 Human Anti-FZD5 Recombinant Antibody (PABJ-0145) in SDS-PAGE
SDS-PAGE analysis of PABJ-0145 in reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
The purity of PABJ-0145 was greater than 95% as determined by SDS-PAGE.
SEC-HPLC
Figure 2 Human Anti-FZD5 Recombinant Antibody (PABJ-0145) in HPLC
The purity of PABJ-0145 was greater than 95% as determined by SEC-HPLC.
WB
Figure 3 Human Anti-FZD5 Recombinant Antibody (PABJ-0145) in WB
Western blot analysis of PABJ-0145 was performed by loading recombinant human FZD5 protein.
PABJ-0145 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-goat anti-human IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
ELISA
Figure 4 Human Anti-FZD5 Recombinant Antibody (PABJ-0145) in ELISA
ELISA analysis of PABJ-0145 was performed by coating with recombinant human FZD5 protein. Then blocked with BSA and incubated with Human Anti-FZD5 Recombinant Antibody (PABJ-0145). The Goat Anti-Human IgG-HRP as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm
❮
❯
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human FZD5
- Species Reactivity
- Human
- Applications
- ELISA, Inhib, FuncS
Product Property
- Purification
- Protein A/G Purified
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Inhibition, Functional Assay.
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for PABJ-0145. Click the button above to contact us or submit your feedback about this product.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABJ-0145, RRID: AB_3111607)
Copy citation
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "FZD5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0749CT | Recombinant Mouse anti-Human FZD5 Monoclonal antibody (EML2242) | WB | |
| MRO-1876-CN | Rabbit Anti-FZD5 Polyclonal Antibody (MRO-1876-CN) | WB | Rabbit IgG |
| VS3-QX453 | Mouse Anti-FZD5 Recombinant Antibody (clone 2D12) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABJ-0145-S(P) | Human Anti-FZD5 Recombinant Antibody scFv Fragment (PABJ-0145-S(P)) | ELISA, Inhib, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABJ-0145-F(E) | Human Anti-FZD5 Recombinant Antibody Fab Fragment (PABJ-0145-F(E)) | ELISA, Inhib, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC416 | Recombinant Anti-FZD5 Vesicular Antibody, EV Displayed (VS-0425-YC416) | ELISA, FC, Inhib, Cell-uptake |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: WB, ELISA, FuncS
Application: SPR, Inhib, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: Neut, ELISA, IF, IP, FuncS, FC
Application: ELISA, IHC, IF, IP
Application: ELISA, Neut
Application: ELISA, WB
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
